产品货号：
GS4637
中文名称：
重组人UBE2B(带His标签)
英文名称：
Recombinant Human Ubiquitin Conjugating Enzyme E2 B, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 19.0kDa,a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a.vector sequence including 6×His tag at N-terminus.
储存条件：冷冻（-20℃）
概述
Ubiquitin-conjugating enzyme E2 B belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2B gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The ubiquitination reaction can modify proteins and regulate protein degradation.The UBE2B interacts with RAD18,UBR2 and WAC.Its protein sequence is 100%identical to the mouse,rat,and rabbit homologs,which indicates that this enzyme is highly conserved in eukaryotic evolution.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人UBE2B(带His标签)，UBE2B, His-tagged, Human
储存条件：冷冻（-20℃）
概述
Ubiquitin-conjugating enzyme E2 B belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2B gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The ubiquitination reaction can modify proteins and regulate protein degradation.The UBE2B interacts with RAD18,UBR2 and WAC.Its protein sequence is 100%identical to the mouse,rat,and rabbit homologs,which indicates that this enzyme is highly conserved in eukaryotic evolution.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Data is not available. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuUBE2B,His as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS |
相关搜索：重组人UBE2B(带His标签)，UBE2B, His-tagged, Human